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  • 26051412PublicAssets/6800A Janus particle being used to activate a T cell, a type of immune cell. A Janus particle is a specialized microparticle with different physical properties on its surface, and this one is coated with nickel on one hemisphere and anti-CD3 antibodies (light blue) on the other. The nickel enables the Janus particle to be moved using a magnet, and the antibodies bind to the T cell and activate it. The T cell in this video was loaded with calcium-sensitive dye to visualize calcium influx, which indicates activation. The intensity of calcium influx was color coded so that warmer color indicates higher intensity. Being able to control Janus particles with simple magnets is a step toward controlling individual cells’ activities without complex magnetic devices.<Br><Br> More details can be found in the <em> Angewandte Chemie </em> paper <a href="https://onlinelibrary.wiley.com/doi/full/10.1002/anie.201601211">“Remote control of T cell activation using magnetic Janus particles”</a> by Lee et al. This video was captured using epi-fluorescence microscopy. <Br><Br>Related to video <a href="https://images.nigms.nih.gov/pages/DetailPage.aspx?imageid2=6801">6801</a>. Yan Yu, Indiana University, Bloomington.Kwahun Lee, Yan Yu Group, Indiana University, Bloomington.Video

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    Magnetic Janus particle activating a T cell

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    A Janus particle being used to activate a T cell, a type of immune cell. A Janus particle is a specialized microparticle with different physical properties on its surface, and this one is coated with nickel on one hemisphere and anti-CD3 antibodies (light blue) on the other. The nickel enables the Janus particle to be moved using a magnet, and the antibodies bind to the T cell and activate it. The T cell in this video was loaded with calcium-sensitive dye to visualize calcium influx, which indicates activation. The intensity of calcium influx was color coded so that warmer color indicates higher intensity. Being able to control Janus particles with simple magnets is a step toward controlling individual cells’ activities without complex magnetic devices.

    More details can be found in the Angewandte Chemie paper “Remote control of T cell activation using magnetic Janus particles” by Lee et al. This video was captured using epi-fluorescence microscopy.

    Related to video 6801.

    Source

    Yan Yu, Indiana University, Bloomington.

    Credit Line

    Kwahun Lee, Yan Yu Group, Indiana University, Bloomington.

    Record Type

    Video

    ID

    6800

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