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View Entry | Spreading Cells- 023329| Cells move forward with lamellipodia and filopodia supported by networks and bundles of actin filaments. Proper, controlled cell movement is a complex process. Recent research has shown that an actin-polymerizing factor called the Arp2/3 complex is the key component of the actin polymerization engine that drives amoeboid cell motility. ARPC3, a component of the Arp2/3 complex, plays a critical role in actin nucleation.
In this photo, the ARPC3-/- fibroblast cells were fixed and stained with Alexa 546 phalloidin for F-actin (red), Arp2 (green), and DAPI to visualize the nucleus (blue). Arp2, a subunit of the Arp2/3 complex, is absent in the filopodi-like structures based leading edge of ARPC3-/- fibroblasts cells.
Related to images 3328, 3330, 3331, 3332, and 3333. | | Public Note | | | | | Internal Note | | Project Overview
The Arp2/3 complex is the key component of the actin polymerization engine that drives amoeboid cell motility. ARPC3, a component of the Arp2/3 complex, plays a critical role in actin nucleation to generate Y-shaped branches from existing actin filaments. It remains unclear whether the Arp2/3 complex is truly important for leading edge extension in amoeboid form of cell motility. We have generated ARPC3+/- mice using a gene-trap ES cell line. To further investigate the function of the Arp2/3 complex in different motile cells we developed an approach to derive ARPC3+/+ and ARPC3-/- ES cells from blastocysts of ARPC3+/- crosses and differentiate them into fibroblasts cells. The deletion of ARPC3, a subunit of the Arp2/3 complex, resulted in a loss of lamellipodia structures. Interestingly, filopodia structures were observed. Cells without lamellipodia were able to migrate with similar speed compared to the wild-type cells. ARPC3-/- fibroblast cells exhibit defect in directional migration.
ARPC3+/+ and ARPC3-/- primary fibroblast cells that are differentiated from the corresponding Mouse Embryonic stem cells.
From: Suraneni, Praveen [PKS@stowers.org]
Sent: Tuesday, April 10, 2012 3:34 PM
To: Jegalian, Karin (NIH/NIGMS) [C]
Subject: RE: On the Move
Hi Jegalian,
Thank you for the request, I can send you few images that are not submitted to JCB. Let me know how many do you need and source to upload them.
Thank you,
Praveen.
From: Li, Rong [mailto:RLI@stowers.org]
Sent: Tuesday, April 10, 2012 11:44 AM
To: Jegalian, Karin (NIH/NIGMS) [C]
Cc: Suraneni, Praveen
Subject: RE: On the Move
Dear Karin,
You are more than welcome to use our images, though I'm unsure about JCB's copyright policy. I imagine that they should be alright with it. We'll be happy to send you the high resolution images too. I'm copying the first author of this email.
Best regards,
Rong | | | Keywords | | | | | Source | | Rong Li and Praveen Suraneni, Stowers Institute for Medical Research | | | Date | | 2012-05-15 00:00:00 | | | Credit Line | | Rong Li and Praveen Suraneni, Stowers Institute for Medical Research | | | Investigator | | Rong Li and Praveen Suraneni | | | Record Type | | Photograph | | | Topic Area(s) | | ;#Cells;# | | | Previous Uses | | | | | Status | | Active | |
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