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    Magnetic Janus particle activating a T cell
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    Magnetic Janus particle activating a T cell

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    A Janus particle being used to activate a T cell, a type of immune cell. A Janus particle is a specialized microparticle with different physical properties on its surface, and this one is coated with nickel on one hemisphere and anti-CD3 antibodies (light blue) on the other. The nickel enables the Janus particle to be moved using a magnet, and the antibodies bind to the T cell and activate it. The T cell in this video was loaded with calcium-sensitive dye to visualize calcium influx, which indicates activation. The intensity of calcium influx was color coded so that warmer color indicates higher intensity. Being able to control Janus particles with simple magnets is a step toward controlling individual cells’ activities without complex magnetic devices.

    More details can be found in the Angewandte Chemie paper “Remote control of T cell activation using magnetic Janus particles” by Lee et al. This video was captured using epi-fluorescence microscopy.

    Related to video 6801.
    Public Note
    Internal NoteAlt text: A sphere with one half colored light blue moves beside a T cell with its light-blue side facing away from the cell. The sphere then rotates so that its light-blue side touches the cell and activates it. The T-cell changes colors from light blue to bright red, then fluctuates between yellow and orange for the rest of the movie. A Janus particle was coated with a thin film of nickel on one hemisphere and functionalized with anti-CD3 (fluorescent) on the other hemisphere. The Janus particle can rotate and move translationally under a rotating magnetic field. When the Janus particle was rotated, its orientation can be used as a switch to turn on the activation of T cells when its anti-CD3 coated hemisphere was rotated to bind to the T cell. The T cell was loaded with the calcium-sensitive dye Fluo-4 to visualize calcium influx, which indicates T cell activation. The intensity of calcium influx was colored coded with warmer color indicating higher intensity. More details can be found in Lee et al. Angew. Chem. Int. Ed. 2016, 55, 7384-7. Dear Abbey, Thank you for reaching out. We will be delighted to provide some videos for the gallery. Here is the link to a folder containing three videos and the corresponding word document with the necessary information. Yan Yu, Ph.D. Associate Professor Chemistry Department Indiana University 800 E. Kirkwood Ave Bloomington, IN 47401 Phone: (812) 855-0593 https://yu.lab.indiana.edu/
    KeywordsImmune system
    SourceYan Yu, Indiana University, Bloomington.
    Date
    Credit LineKwahun Lee, Yan Yu Group, Indiana University, Bloomington.
    Investigator
    Record TypeVideo
    Topic Area(s);#Cells;#Tools and Techniques;#
    Previous Uses
    StatusActive

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