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  • 162241099244747995811254966580251405627481431124PublicAssets/6780The green spots in this mouse brain are cells labeled with Calling Cards, a technology that records molecular events in brain cells as they mature. Understanding these processes during healthy development can guide further research into what goes wrong in cases of neuropsychiatric disorders. Also fluorescently labeled in this image are neurons (red) and nuclei (blue). Calling Cards and its application are described in the <em>Cell</em> paper “<a href=https://www.sciencedirect.com/science/article/pii/S009286742030814X>Self-Reporting Transposons Enable Simultaneous Readout of Gene Expression and Transcription Factor Binding in Single Cells</a>” by Moudgil et al.; and the <em>Proceedings of the National Academy of Sciences</em> paper “<a href=https://www.pnas.org/content/117/18/10003>A viral toolkit for recording transcription factor–DNA interactions in live mouse tissues</a>” by Cammack et al. The technology was also featured in the <em>NIH Director’s Blog</em> post <a href=https://directorsblog.nih.gov/2021/08/24/the-amazing-brain-tracking-molecular-events-with-calling-cards-in-the-living-brain/>The Amazing Brain: Tracking Molecular Events with Calling Cards</a>. <Br><Br> Related to video <a href=https://images.nigms.nih.gov/pages/DetailPage.aspx?imageid2=6781>6781</a>.Allen Yen, Lab of Joseph Dougherty, Washington University School of Medicine in St. Louis.Allen Yen, Lab of Joseph Dougherty, Washington University School of Medicine in St. Louis.Photograph

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    Calling Cards in a mouse brain

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    The green spots in this mouse brain are cells labeled with Calling Cards, a technology that records molecular events in brain cells as they mature. Understanding these processes during healthy development can guide further research into what goes wrong in cases of neuropsychiatric disorders. Also fluorescently labeled in this image are neurons (red) and nuclei (blue). Calling Cards and its application are described in the Cell paper “Self-Reporting Transposons Enable Simultaneous Readout of Gene Expression and Transcription Factor Binding in Single Cells” by Moudgil et al.; and the Proceedings of the National Academy of Sciences paper “A viral toolkit for recording transcription factor–DNA interactions in live mouse tissues” by Cammack et al. The technology was also featured in the NIH Director’s Blog post The Amazing Brain: Tracking Molecular Events with Calling Cards.

    Related to video 6781.

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    Allen Yen, Lab of Joseph Dougherty, Washington University School of Medicine in St. Louis.

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    Allen Yen, Lab of Joseph Dougherty, Washington University School of Medicine in St. Louis.

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    Photograph

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    6780

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