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Confocal image showing high levels of the protein vimentin (white) at the edge zone of a quail embryo. Cell nuclei are labeled green.
More specifically, this high-magnification (60X) image shows vimentin immunofluorescence in the edge zone (top of image) and inner zone (bottom of image) of a Stage 4 quail blastoderm. Vimentin expression (white) is shown merged with Sytox nuclear labeling (green) at the edge of the blastoderm. A thick vimentin filament runs circumferentially (parallel to the direction of the edge) that appears to delineate the transition between the edge zone and interior zone. Also shown are dense vimentin clusters or foci, which typically appear to be closely associated with edge cell nuclei.
An NIGMS grant to Professor Garcia was used to purchase the confocal microscope that collected this image. Related to image 2808 and video 2809.
Andrés Garcia, Georgia Tech
Evan Zamir, Georgia Tech
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