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    Fruit fly ovarioles
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    Fruit fly ovarioles

    6810

    Three fruit fly (Drosophila melanogaster) ovarioles (yellow, blue, and magenta) with egg cells visible inside them. Ovarioles are tubes in the reproductive systems of female insects. Egg cells form at one end of an ovariole and complete their development as they reach the other end, as shown in the yellow wild-type ovariole. This process requires an important protein that is missing in the blue and magenta ovarioles. This image was created using confocal microscopy.

    More information on the research that produced this image can be found in the Current Biology paper “Gatekeeper function for Short stop at the ring canals of the Drosophila ovary” by Lu et al.
    Public Note
    Internal NoteAlt text: Three stacks of ovals: one yellow, one blue, and one purple. Inside the ovals are smaller round shapes. In the background are two large ovals filled with white strings and black spots. Researcher-Provided Description: Drosophila melanogaster (fruit fly) oocyte growth requires an actin-microtubule cross-linker, short stop (shot). The color images are wild-type (yellow) and two shot-RNAi lines (cyan and magenta) in which actin filaments (labeled with rhodamine-conjugated phalloidin) were used to outline the cell shape of oocytes. The black-and-white image shows microtubules and actin filaments in a shot-RNAi background. The color images were acquired on a Nikon A1plus scanning confocal microscope with a GaAsP detector and a 20X 0.75 N.A. lens using Galvano scanning, and the black-and-white image was acquired on a Nikon W1 spinning disk confocal microscope (Yokogawa CSU with pinhole size 50 µm) with a Photometrics Prime 95B sCMOS Camera and a 40X 1.25 N.A. silicone oil lens, controlled by Nikon Elements software. Dear Abbey, We have some images for you. Would you like to have a look at them? How do you want me to share them with you (TIFF files are too big for email). Best, Vladimir Gelfand
    KeywordsOocytes, oogenesis, research organisms
    SourceVladimir I. Gelfand, Feinberg School of Medicine, Northwestern University.
    Date
    Credit LineWen Lu and Vladimir I. Gelfand, Feinberg School of Medicine, Northwestern University.
    Investigator
    Record TypePhotograph
    Topic Area(s);#Cells;#Genes;#Tools and Techniques;#
    Previous Uses
    StatusActive

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Medium290 KB 2/11/2022 2:16 PMDolan, Lauren (NIH/NIGMS) [C]
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