• 21976345PublicAssets/6781
    Video of Calling Cards in a mouse brain
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    Video of Calling Cards in a mouse brain

    6781

    The green spots in this mouse brain are cells labeled with Calling Cards, a technology that records molecular events in brain cells as they mature. Understanding these processes during healthy development can guide further research into what goes wrong in cases of neuropsychiatric disorders. Also fluorescently labeled in this video are neurons (red) and nuclei (blue). Calling Cards and its application are described in the Cell paper “Self-Reporting Transposons Enable Simultaneous Readout of Gene Expression and Transcription Factor Binding in Single Cells” by Moudgil et al.; and the Proceedings of the National Academy of Sciences paper “A viral toolkit for recording transcription factor–DNA interactions in live mouse tissues” by Cammack et al. This video was created for the NIH Director’s Blog post The Amazing Brain: Tracking Molecular Events with Calling Cards.

    Related to image 6780.
    Public Note
    Internal NoteHi Abbey et. al – I see that Drs. Yen, Dougherty, and Mitra have given their permission. The image belongs to them, and it was Dr. Collins’ pleasure to have their permission to feature it and the excellent science on his blog. Please feel free to use the image, as modified by Donny into a video. We would just ask that NIGMS also give credit to the NIH Director’s Blog and add a link/reference to the blog post, where your readers could get more information about the science.
    Keywordsnerve cells, neuroscience
    SourceNIH Director’s Blog.
    Date
    Credit LineAllen Yen, Lab of Joseph Dougherty, Washington University School of Medicine in St. Louis; and NIH Director’s Blog.
    InvestigatorGM Grantee Robi Mitra: https://reporter.nih.gov/search/YEUu88ffvkCI2g5oFvLR_g/project-details/10155502
    Record TypeVideo
    Topic Area(s);#Cells;#Tools and Techniques;#
    Previous Uses
    StatusActive

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mouse-brain-2-720_mp4_hd.mp4
  
Other21462 KB 9/10/2021 1:14 PMDolan, Lauren (NIH/NIGMS) [C]
MouseBrainVideo_Thumb.png
  
Thumbnail3014 KB 9/10/2021 1:10 PMDolan, Lauren (NIH/NIGMS) [C]

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