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    Cell-like compartments from frog eggs 5
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    Cell-like compartments from frog eggs 5

    6592

    Cell-like compartments that spontaneously emerged from scrambled frog eggs, with nuclei (blue) from frog sperm. Endoplasmic reticulum (red) and microtubules (green) are also visible. Image created using confocal microscopy.

    For more photos of cell-like compartments from frog eggs view: 6584, 6585, 6586, 6591, and 6593.

    For videos of cell-like compartments from frog eggs view: 6587, 6588, 6589, and 6590.

    Public Note
    Internal NoteFrog sperm nuclei were added to these egg extracts to provide a source of nuclei, but they are not required for compartment formation. Sperm nuclei-containing compartments can divide repeatedly to produce more compartments like cells in an embryo. Shown in blue are the nuclei, visualized by the fluorescent protein mCherry-NLS. Shown in red is the endoplasmic reticulum, stained by the ER-Tracker dye. Shown in green are the microtubules, visualized by SiR-tubulin stain. The image was acquired on a Nikon Eclipse-TI inverted spinning disk confocal microscope with a 20x objective. It captures a 1.04 mm by 0.66 mm rectangular region. News coverage: Stanford Medicine press releaseQuanta MagazineStanford Scope Blog
    Keywordsstructure
    SourceXianrui Cheng, Stanford University School of Medicine.
    Date
    Credit LineXianrui Cheng, Stanford University School of Medicine. Xianrui Cheng, James E. Ferrell Jr. SCIENCE 366: 631, 01 Nov 2019 (DOI: 10.1126/science.aav7793)
    Investigator
    Record TypePhotograph
    Topic Area(s);#Cells;#Tools and Techniques;#
    Previous Uses
    StatusActive

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Medium66 KB 9/15/2020 10:07 AMVarkala, Venkat (NIH/NIGMS) [C]
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