• 20001469183331099973411911249936722965PublicAssets/6572
    Nuclear Lamina
    Active
    View Entry

    Large-Resolution Image
    2000 x 1469 pixels
    7 × 5 inches (300 dpi)
    1.75Mb

    Medium-Resolution Image
    999 x 734 pixels
    7 × 5 inches (150 dpi)
    116.3kb

    Low-Resolution Image
    499 x 367 pixels
    7 × 5 inches (72 dpi)
    22.4kb

    Nuclear Lamina

    6572

    The 3D single-molecule super-resolution reconstruction of the entire nuclear lamina in a HeLa cell was acquired using the TILT3D platform. TILT3D combines a tilted light sheet with point-spread function (PSF) engineering to provide a flexible imaging platform for 3D single-molecule super-resolution imaging in mammalian cells.
    See 6573 for 3 seperate views of this structure.
    Public Note
    Internal Notepermission email: https://iqsolutions.sharepoint.com/:f:/t/NIGMS-P/EsN3sXoMRAtKtxlbk8x5yzkBPJSSFwqzLSy7nQ9doQnJAQ?e=EnClBJ Private gallery notes: Here, lamin B1 was immunolabeled with Alexa Fluor 647. Single molecules were detected in several thick, overlapping slices using the double-helix PSF, while fiducial beads were detected using a 6-μm Tetrapod PSF. For more information, please see: Gustavsson et al. 3D single-molecule super-resolution microscopy with a tilted light sheet, Nat. Commun. 9(123) 2018. https://doi.org/10.1038/s41467-017-02563-4 Gustavsson et al. Light sheet approaches for improved precision in 3D localization-based super-resolution imaging in mammalian cells, Opt. Express 26(10) 2018
    https://doi.org/10.1364/OE.26.013122
    https://news.stanford.edu/2018/02/22/seeing-nanoscale-details-mammalian-cells/
    http://news.rice.edu/2020/02/19/cprit-grant-draws-cell-imaging-specialist-to-rice-2/
    Keywordsnucleus
    SourceAnna-Karin Gustavsson, Ph.D.
    Date
    Credit LineAnna-Karin Gustavsson (Department of Chemistry, Stanford University and Department of Biosciences and Nutrition, Karolinska Institutet), Petar N. Petrov (Department of Chemistry, Stanford University), Maurice Y. Lee (Department of Chemistry and Biophysics Program, Stanford University), Yoav Shechtman (Biomedical Engineering Department, Technion, Israel, Institute of Technology), and W. E. Moerner (Department of Chemistry and Biophysics Program, Stanford University).
    Investigator
    Record TypePhotograph
    Topic Area(s);#Cells;#Molecular Structures;#Tools and Techniques;#
    Previous Uses
    StatusActive

    View All Properties
    Edit Properties
  
  
  
  
  
  
NuclearLamina_300dpi_M.jpg
  
Medium117 KB 7/16/2020 5:42 PMHarris, Donald (NIH/NIGMS) [C]
NuclearLamina_300dpi_S.jpg
  
Low23 KB 7/16/2020 5:42 PMHarris, Donald (NIH/NIGMS) [C]
NuclearLamina_300dpi_thumbnail.jpg
  
Thumbnail2 KB 7/16/2020 5:42 PMHarris, Donald (NIH/NIGMS) [C]
NuclearLamina_300dpi.jpg
  
High1791 KB 7/16/2020 5:42 PMHarris, Donald (NIH/NIGMS) [C]

 Add New Version

Note: Uploading a version which already exists will overwrite the existing version with the uploaded file.
* Uploaded thumbnails wider than 120 pixels will be reduced.