• 6664666431186084300030003327299308308169818PublicAssets/5838
    Color coding of the Drosophila brain - image
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    Color coding of the Drosophila brain - image

    5838

    This image results from a research project to visualize which regions of the adult fruit fly (Drosophila) brain derive from each neural stem cell. First, researchers collected several thousand fruit fly larvae and fluorescently stained a random stem cell in the brain of each. The idea was to create a population of larvae in which each of the 100 or so neural stem cells was labeled at least once. When the larvae grew to adults, the researchers examined the flies’ brains using confocal microscopy. With this technique, the part of a fly’s brain that derived from a single, labeled stem cell “lights up. The scientists photographed each brain and digitally colorized its lit-up area. By combining thousands of such photos, they created a three-dimensional, color-coded map that shows which part of the Drosophila brain comes from each of its ~100 neural stem cells. In other words, each colored region shows which neurons are the progeny or “clones” of a single stem cell. This work established a hierarchical structure as well as nomenclature for the neurons in the Drosophila brain. Further research will relate functions to structures of the brain.

    Related to image 5868 and video 5843
    Public Note
    Internal NoteAcknowledgements: Dr. Kei Ito’s group at the University of Tokyo prepared the biological samples and generated the Drosophila brain data set. FluoRender, developed at the SCI Institute, University of Utah, was used for the visualizations. A 96-channel volumetric Drosophila brain atlas was presented with all its fine details thanks to the collaborations among researchers in biology and computer technologies. Reference: Ito, M., Masuda, N., Shinomiya, K., Endo, K. & Ito, K. (2013). Systematic analysis of neural projections reveals clonal composition of the Drosophila brain. Current Biology, 23(8), 644-655.

    From Yong Wan (wanyong@cs.utah.edu) The software (FluoRender) was initially funded with the R01 GM98151-04 to Charles Hansen (PD Jim Deatherage). That grant ended but now the software is funded through the BTRR to Chris Johnson. Additional Reference: Wan, Y., Otsuna, H., Holman, H. A., Bagley, B., Ito, M., Lewis, A. K., Colasanto, M., Kardon, G., Ito, K. & Hansen, C. FluoRender: joint free-hand segmentation and visualization for many-channel fluorescence data analysis. Manuscript submitted to BMC Bioinformatics
    KeywordsDrosophila Fruit fly BTRR Brain
    SourceYong Wan from Charles Hansen’s lab, University of Utah. Data preparation and visualization by Masayoshi Ito in the lab of Kei Ito, University of Tokyo.
    Date
    Credit LineYong Wan, Charles Hansen and Chris R. Johnson, University of Utah
    InvestigatorChris R. Johnson and Charles (“Chuck”) Hansen, University of Utah
    Record TypePhotograph
    Topic Area(s);#Cells;#Tools and Techniques;#
    Previous UsesStrathmore Mansion 2017
    StatusActive

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5838_droso_x10_crop from Utah BTRR--Chris Johnson_S.jpg
  
Low166 KB 3/28/2019 2:31 PMConstantinides, Stephen (NIH/NIGMS) [C]
5838_droso_x10_crop from Utah BTRR--Chris Johnson_T.jpg
  
Thumbnail166 KB 3/28/2019 2:31 PMConstantinides, Stephen (NIH/NIGMS) [C]
droso_x10_crop-M.jpg
  
Medium3250 KB 2/8/2017 9:44 AMMachalek, Alisa (NIH/NIAMS) [E]
droso_x10_crop from Utah BTRR--Chris Johnson_ PI.tiff
  
High30456 KB 2/7/2017 12:09 PMBarrat, Hunter (NIH/NIGMS) [C]

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