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    Nucleolus subcompartments spontaneously self-assemble 4
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    Nucleolus subcompartments spontaneously self-assemble 4


    What looks a little like distant planets with some mysterious surface features are actually assemblies of proteins normally found in the cell's nucleolus, a small but very important protein complex located in the cell's nucleus. It forms on the chromosomes at the location where the genes for the RNAs are that make up the structure of the ribosome, the indispensable cellular machine that makes proteins from messenger RNAs.

    However, how the nucleolus grows and maintains its structure has puzzled scientists for some time. It turns out that even though it looks like a simple liquid blob, it's rather well-organized, consisting of three distinct layers: the fibrillar center, where the RNA polymerase is active; the dense fibrillar component, which is enriched in the protein fibrillarin; and the granular component, which contains a protein called nucleophosmin. Researchers have now discovered that this multilayer structure of the nucleolus arises from differences in how the proteins in each compartment mix with water and with each other. These differences let the proteins readily separate from each other into the three nucleolus compartments.

    This photo of nucleolus proteins in the eggs of a commonly used lab animal, the frog Xenopus laevis, shows each of the nucleolus compartments (the granular component is shown in red, the fibrillarin in yellow-green, and the fibrillar center in blue). The researchers have found that these compartments spontaneously fuse with each other on encounter without mixing with the other compartments.

    For more details on this research, see this press release from Princeton. Related to video 3789, video 3791 and image 3792.
    Public Note
    Internal NoteResearchers supplied video and gave permission for public use: From: Nilesh Vaidya [nvaidya@princeton.edu] To: Spiering, Martin (NIH/NIGMS) [C] Cc: Clifford P. Brangwynne [cbrangwy@princeton.edu] Thursday, May 26, 2016 10:33 AM Dear Martin, I am a co-first author of the article published on the journal Cell on how the nucleolus self-organizes. I was informed by my advisor Cliff Brangwynne that you are interested in including some of the images and videos of the nucleolus reported in our paper in your image gallery. I have attached few images (two for native and two for drug treated nucleoli) and couple of movies (fusion of nucleoli following drug treatment) with this email that are not published. For all images and movies, granular component (GC) is visualized with NPM1 (red), dense fibrillar component (DFC) with FIB1 (green), and fibrillar center (FC) with POLR1E (blue). Please let me know if you have any questions. Thank you. Best, Nilesh Vaidya Postdoctoral fellow Brangwynne lab Chemical and Biological Engineering Princeton University From: "Spiering, Martin (NIH/NIGMS) [C]" Subject: Videos/images of nucleolus for NIGMS image gallery? Date: May 23, 2016 at 12:37:21 PM EDT To: "cbrangwy@princeton.edu" Dear Dr. Brangwynne, I am a writer and editor with the Office of Communication and Public Liaison at the National Institute of General Medical Sciences. I'm reaching out to you because we noticed your exciting new findings of how the nucleolus self-organizes, which were reported in a recent news release (athttp://www.princeton.edu/main/news/archive/S46/35/80M01/?section=topstories) highlighting your work. We're very interested in including some of your striking images and videos of the nucleolus reported in the paper into our image gallery (at https://images.nigms.nih.gov/) to bring your important work to broader public attention.
    SourceNilesh Vaidya, Princeton University
    Credit LineNilesh Vaidya and Clifford Brangwynne, Princeton University
    InvestigatorClifford Brangwynne, Princeton University
    Record TypePhotograph
    Topic Area(s);#Cells;#Genes;#
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