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    Active site of sulfite oxidase

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    Active site of sulfite oxidase


    Sulfite oxidase is an enzyme that is essential for normal neurological development in children. This video shows the active site of the enzyme and its molybdenum cofactor visible as a faint ball-and-stick representation buried within the protein. The positively charged channel (blue) at the active site contains a chloride ion (green) and three water molecules (red). As the protein oscillates, one can see directly down the positively charged channel. At the bottom is the molybdenum atom of the active site (light blue) and its oxo group (red) that is transferred to sulfite to form sulfate in the catalytic reaction.
    Public Note
    Internal NoteJE offered video to AZM 6/10/2010: Our research on molybdenum enzymes, particularly sulfite oxidase, is supported under grant GM-037773. I have presented this video in seminars and at conferences, but it has not been published anywhere. The coordinates for calculating this video come from the crystal structure of the Mo Domain of recombinant chicken sulfite oxidase (PDB 2A99; E. Karakas, H. L. Wilson, T. N. Graf, S. Xiang, S. Jaramillo-Busquets, K. V. Rajagopalan and C. Kisker, J. Biol. Chem., 2005, 280, 33506?33515). The heme domain is somehow lost during preparation or crystallization, but the structure of the Mo domain is very similar to the native intact chicken enzyme (PDB 1SOX; C. Kisker, H. Schindelin, A. Pacheco, W. A. Wehbi, R. M. Garrett, K. V. Rajagopalan, J. H. Enemark and D. C. Rees, Cell, 1997, 91, 973?983). The chicken enzyme has high sequence homology to the human enzyme, which has not been successfully crystallized.
    SourceJohn Enemark, University of Arizona
    Date2010-06-11 00:00:00
    Credit LineJohn Enemark, University of Arizona
    InvestigatorJohn Enemark, University of Arizona
    Record TypeVideo
    Topic Area(s);#Cells;#Chemistry, Biochemistry, and Pharmacology;#
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Thumbnail815 KB 6/21/2016 11:25 AMaavarkalavr (NIH/NIGMS) [C]
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