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    Shiga toxin
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    Shiga toxin


    E. coli bacteria normally live harmlessly in our intestines, but some cause disease by making toxins. One of these toxins, called Shiga toxin (green), inactivates host ribosomes (purple) by mimicking their normal binding partners, the EF-Tu elongation factor (red) complexed with Phe-tRNAPhe (orange).

    Find these in the RCSB Protein Data Bank: Shiga toxin 2 (PDB entry 7U6V) and Phe-tRNA (PDB entry 1TTT).

    More information about this work can be found in the J. Biol. Chem. paper "Cryo-EM structure of Shiga toxin 2 in complex with the native ribosomal P-stalk reveals residues involved in the binding interaction" by Kulczyk et. al.
    Public NoteA dark red orb with bright red projections off the bottom. One is attached to a green blob, and other green and orange blobs are floating around it. Green cylinders are in the bottom.
    Internal NoteFrom: Amy Wu Sent: Thursday, February 1, 2024 4:20 PM To: Bigler, Abbey (NIH/NIGMS) [C] ; Crowley, Rachel (NIH/NIGMS) [E] Cc: Christine Zardecki Subject: [EXTERNAL] RCSB PDB Content for the NIGMS Image and Video Gallery Hi! I hope all is well. Attached is a Google Folder link + Word document with all the requested images and information. Link: https://drive.google.com/drive/folders/17GbO9ddzOn0UAifMihHDo6qAQdIQgE4r?usp=drive_link Please feel free to email me with any questions. Sincerely, Amy Funding Source: RCSB PDB Core Operations are funded by the National Science Foundation (DBI-1832184), the US Department of Energy (DE-SC0019749), and the National Cancer Institute, National Institute of Allergy and Infectious Diseases, and National Institute of General Medical Sciences of the National Institutes of Health under grant R01GM133198.
    KeywordsEscherichia coli
    SourceAmy Wu and Christine Zardecki, RCSB Protein Data Bank.
    Credit LineCreated by Maria Voigt of the RCSB Protein Data Bank with materials provided by Arek Kulczyk of Rutgers University, Institute for Quantitative Biomedicine.
    InvestigatorTitle: Shiga toxin 2 in complex with ribosomal P-stalk. Some of bacterial ribosome-inactivating toxins evolved to mimic the mechanism employed for loading onto the ribosome by translation factors. The illustration depicts how Shiga toxin 2a (Stx2a) holotoxin (green, PDB ID: 7U6V, EMD-26381) outcompetes the EF-Tu elongation factor (red, PDB ID: 1TTT) complexed with Phe-tRNAPhe (orange) for binding to the ribosomal P-stalk (red) at the large subunit of the 80S ribosome (purple, EMD-26380). Kulczyk et al. determined a cryo-EM structure of Stx2a bound to the native ribosomal P-stalk. Sourced some info from the CDC site: https://www.cdc.gov/ecoli/general/index.html
    Record TypeIllustration
    Topic Area(s);#Injury and Illness;#Molecular Structures;#
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Medium101 KB 2/2/2024 3:31 PMCrowley, Rachel (NIH/NIGMS) [E]
Thumbnail2 KB 2/2/2024 3:31 PMCrowley, Rachel (NIH/NIGMS) [E]
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High3554 KB 2/2/2024 3:31 PMCrowley, Rachel (NIH/NIGMS) [E]

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