• 1190605248010816594302295032971516835PublicAssets/6983
    Genetic mosaicism in fruit flies
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    Genetic mosaicism in fruit flies

    6983

    Fat tissue from the abdomen of a genetically mosaic adult fruit fly. Genetic mosaicism means that the fly has cells with different genotypes even though it formed from a single zygote. This specific mosaicism results in accumulation of a critical fly adipokine (blue-green) within the fat tissue cells that have reduced expression a key nutrient sensing gene (in left panel). The dotted line shows the cells lacking the gene that is present and functioning in the rest of the cells. Nuclei are labelled in magenta. This image was captured using a confocal microscope and shows a maximum intensity projection of many slices.

    Related to images 6982, 6984, and 6985.
    Public NoteLeft, blue dots outlined in yellow; right, blue-green accumulation in the yellow outline but not outside of the outline.
    Internal NoteGrant R35GM124593 Hi Rachel, Thanks for your patience. Since the images are too large to attach I have uploaded them at this google drive link and you should be able to download it the link. You would also have received an email from my google drive with this link. Please let me know if you have any difficulties. Here are snapshots of the 4 images and attached word doc has the caption, and necessary credit. Again, thanks for reaching out to feature some of our work in the NIGMS image gallery as a resource. Best wishes, Akhila
    Keywords
    SourceAkhila Rajan, Fred Hutchinson Cancer Center
    Date
    Credit LineAditi Madan, Akhila Rajan’s lab, Fred Hutchinson Cancer Center
    InvestigatorA genetically mosaic Drosophila adult fat tissue from the fly abdomen. This results in accumulation of a critical fly adipokine (blue-green) within the fat tissue cells that have a have a gene knockdown for a key nutrient sensing gene (in left panel). The dotted line shows the mutant cells and the rest of the cells are normal. Image was captured using a confocal and a maximum intensity projection of many slices is shown.
    Record TypePhotograph
    Topic Area(s);#Cells;#Chemistry, Biochemistry, and Pharmacology;#Genes;#Tools and Techniques;#
    Previous Usespictures from the 2023 Greenberg lecture: https://www.nigms.nih.gov/News/meetings/Pages/2023-greenberg-eci-lecture.aspx
    StatusActive

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#2 Clonal analysis - Atg8KD_NIGMS.tif
  
High242199 KB 12/19/2023 4:30 PMCrowley, Rachel (NIH/NIGMS) [E]
2 thumbnail.png
  
Thumbnail100 KB 12/19/2023 4:24 PMCrowley, Rachel (NIH/NIGMS) [E]
#2 Clonal analysis - Atg8KD_NIGMS_M.jpg
  
Medium29 KB 12/19/2023 2:18 PMCrowley, Rachel (NIH/NIGMS) [E]
#2 Clonal analysis - Atg8KD_NIGMS_S.jpg
  
Low7 KB 12/19/2023 2:18 PMCrowley, Rachel (NIH/NIGMS) [E]

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