• 1021102114388385105105728151051057281PublicAssets/6970
    Snowflake yeast
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    Snowflake yeast

    6970

    Multicellular yeast called snowflake yeast that researchers created through many generations of directed evolution from unicellular yeast. Cells are connected to one another by their cell walls, shown in blue. Stained cytoplasm (green) and membranes (magenta) show that the individual cells remain separate. This image was captured using spinning disk confocal microscopy.

    Related to images 6969 and 6971.
    Public NoteMany long ovals outlined in blue and magenta and connected to one another.
    Internal NoteFrom: Ratcliff, William C william.ratcliff@biology.gatech.edu Sent: Tuesday, January 3, 2023 4:16 PM To: Kimberly Rousseau krousseau@iqsolutions.com Cc: Tony Burnetti tony.burnetti@gmail.com; Burnetti, Anthony J anthony.burnetti@biosci.gatech.edu Subject: Re: For Review: NIGMS Blog Post

    CAUTION: This email originated from an external sender

    Hi Kim,

    Great! Looking forward to reading the final piece.

    I'm more than happy to add these images to the NIGMS gallery! These images have not been used in any papers yet, but I was planning on submitting them as journal cover art. I assume that's still OK if they're in the NIGMS gallery first. If not, I'll tell the journals to shove it. They can't hold copyright over our images.

    Credit information: Image by Anthony Burnetti, Ozan Bozdag and Will Ratcliff, Georgia Institute of Techology.

    As for the microscopy details, I will let Tony answer this. He is the one who took the pictures.

    Tony, can you do me a favor and write a brief title / caption for these images?

    Cheers,

    Will

    ps- Happy New Year!

    Associate Professor, Biological Sciences Director, Interdisciplinary Graduate Program in Quantitative Biosciences (QBioS) Georgia Institute of Technology Lab website: http://www.ratclifflab.biology.gatech.edu/ Google Scholar profile Twitter: @wc_ratcliff Phone: 612-840-4983 Office: 331 Cherry Emerson Lab: 330 Cherry Emerson
    KeywordsResearch organisms, model organisms, saccharomyces cerevisiae
    SourceWilliam Ratcliff, Georgia Institute of Technology.
    Date
    Credit LineAnthony Burnetti, Ozan Bozdağ, and William Ratcliff, Georgia Institute of Technology.
    InvestigatorStains and fluorescent proteins show the structure of large multicellular yeast clusters after 600 days of directed evolution for large size. Highly elongated cells are connected to each other by their cell walls (blue). Green fluorescent protein and membrane stains (red) reveal that even after hundreds of days of evolution leading to macroscopic growth forms, individual cells remain separate unlike in most multicellular fungi.

    The cytoplasm is tagged with GFP, hence gaps within the cells where vacuoles are holding fluid that is not cytoplasm.

    There does appear to be lots of overlap between the cell wall and membrane stains at this low magnification, but I believe I should probably indeed have called that channel magenta rather than red.
    Record TypePhotograph
    Topic Area(s);#Cells;#Tools and Techniques;#
    Previous UsesGrant info: R35GM138030
    StatusActive

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Snowflake Yeast 2_S.jpg
  
Low56 KB 2/3/2023 5:02 PMBigler, Abbey (NIH/NIGMS) [C]
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Thumbnail3 KB 2/3/2023 4:54 PMBigler, Abbey (NIH/NIGMS) [C]
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High1406 KB 2/3/2023 4:54 PMBigler, Abbey (NIH/NIGMS) [C]

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