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View Entry | Snowflake yeast6970Multicellular yeast called snowflake yeast that researchers created through many generations of directed evolution from unicellular yeast. Cells are connected to one another by their cell walls, shown in blue. Stained cytoplasm (green) and membranes (magenta) show that the individual cells remain separate. This image was captured using spinning disk confocal microscopy.
Related to images 6969 and 6971. | | Public Note | | Many long ovals outlined in blue and magenta and connected to one another. | | | Internal Note | | From: Ratcliff, William C william.ratcliff@biology.gatech.edu
Sent: Tuesday, January 3, 2023 4:16 PM
To: Kimberly Rousseau krousseau@iqsolutions.com
Cc: Tony Burnetti tony.burnetti@gmail.com; Burnetti, Anthony J anthony.burnetti@biosci.gatech.edu
Subject: Re: For Review: NIGMS Blog Post
CAUTION: This email originated from an external sender
Hi Kim,
Great! Looking forward to reading the final piece.
I'm more than happy to add these images to the NIGMS gallery! These images have not been used in any papers yet, but I was planning on submitting them as journal cover art. I assume that's still OK if they're in the NIGMS gallery first. If not, I'll tell the journals to shove it. They can't hold copyright over our images.
Credit information: Image by Anthony Burnetti, Ozan Bozdag and Will Ratcliff, Georgia Institute of Techology.
As for the microscopy details, I will let Tony answer this. He is the one who took the pictures.
Tony, can you do me a favor and write a brief title / caption for these images?
Cheers,
Will
ps- Happy New Year!
Associate Professor, Biological Sciences
Director, Interdisciplinary Graduate Program in Quantitative Biosciences (QBioS)
Georgia Institute of Technology
Lab website: http://www.ratclifflab.biology.gatech.edu/
Google Scholar profile
Twitter: @wc_ratcliff
Phone: 612-840-4983
Office: 331 Cherry Emerson
Lab: 330 Cherry Emerson | | | Keywords | | Research organisms, model organisms, saccharomyces cerevisiae | | | Source | | William Ratcliff, Georgia Institute of Technology. | | | Date | | | | | Credit Line | | Anthony Burnetti, Ozan Bozdağ, and William Ratcliff, Georgia Institute of Technology. | | | Investigator | | Stains and fluorescent proteins show the structure of large multicellular yeast clusters after 600 days of directed evolution for large size. Highly elongated cells are connected to each other by their cell walls (blue). Green fluorescent protein and membrane stains (red) reveal that even after hundreds of days of evolution leading to macroscopic growth forms, individual cells remain separate unlike in most multicellular fungi.
The cytoplasm is tagged with GFP, hence gaps within the cells where vacuoles are holding fluid that is not cytoplasm.
There does appear to be lots of overlap between the cell wall and membrane stains at this low magnification, but I believe I should probably indeed have called that channel magenta rather than red. | | | Record Type | | Photograph | | | Topic Area(s) | | ;#Cells;#Tools and Techniques;# | | | Previous Uses | | Grant info: R35GM138030 | | | Status | | Active | |
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