• 15559688PublicAssets/6898
    Crane fly spermatocyte undergoing meiosis
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    Crane fly spermatocyte undergoing meiosis

    6898

    A crane fly spermatocyte during metaphase of meiosis-I, a step in the production of sperm. A meiotic spindle pulls apart three pairs of autosomal chromosomes, along with a sex chromosome on the right. Tubular mitochondria surround the spindle and chromosomes. This video was captured with quantitative orientation-independent differential interference contrast and is a time lapse showing a 1-second image taken every 30 seconds over the course of 30 minutes.

    More information about the research that produced this video can be found in the J. Biomed Opt. paper “Orientation-Independent Differential Interference Contrast (DIC) Microscopy and Its Combination with Orientation-Independent Polarization System” by Shribak et. al.
    Public NoteA black and white video showing a cell dividing, with chromosomes being pulled apart at the spindle.
    Internal NoteYes, I give my permission to add the three movies (crane fly, circular lamellipodia, and baby squids) and the mouse brain slice image to your image and video gallery. Thank you, Michael Michael Shribak, Ph.D. Senior Scientist Marine Biological Laboratory/ University of Chicago 7 MBL St Woods Hole, MA 02543
    Keywordscell division
    SourceMichael Shribak, Marine Biological Laboratory/University of Chicago.
    Date
    Credit LineMichael Shribak, Marine Biological Laboratory/University of Chicago, and James LaFountain, University at Buffalo.
    InvestigatorThe movie shows an example of the OI-DIC image of a crane fly spermatocyte during metaphase of meiosis-I, taken with a 100x/1.30 oil immersion objective lens. The three autosomal, bivalent chromosomes are pulled apart at the spindle equator, along with one of the X-Y sex univalents located at the right. The distribution of tubular mitochondria surrounding the spindle and granual chromosome structure is clearly visible. The experiment was done by M. Shribak and J. R. LaFountain, Jr. (University at Buffalo, Buffalo, NY) (Shribak et al., 2008). David Biggs, of KB Imaging Solutions LLC, Loomis, CA, computed the optical path difference (OPD) image, using iterative deconvolution algorithms. Image acquisition and processing took about 1 second each. Image size is 68 µm x68 µm. We took one OPD image each 30 seconds. Captured with quantitative orientation-independent differential interference contrast (OI-DIC). https://www.ncbi.nlm.nih.gov/pmc/articles/PMC2302836/ The overall time frame of this time-lapse video is 30 min. https://figshare.com/articles/media/Crane_Fly_movie_avi/6483647
    Record TypeVideo
    Topic Area(s);#Cells;#Molecular Structures;#Tools and Techniques;#
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    StatusActive

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