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    Phagosome in macrophage cell
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    Phagosome in macrophage cell

    6799

    A sensor particle being engulfed by a macrophage—an immune cell—and encapsuled in a compartment called a phagosome. The phagosome then fuses with lysosomes—another type of compartment. The left video shows snowman-shaped sensor particles with fluorescent green nanoparticle “heads” and “bodies” colored red by Förster Resonance Energy Transfer (FRET)-donor fluorophores. The middle video visualizes light blue FRET signals that are only generated when the “snowman” sensor—the FRET-donor—fuses with the lysosomes, which are loaded with FRET-acceptors. The right video combines the other two. The videos were captured using epi-fluorescence microscopy.

    More details can be found in the paper “Transport motility of phagosomes on actin and microtubules regulates timing and kinetics of their maturation” by Yu et al.
    Public Note
    Internal NoteAlt text: On the left, several snowman-shaped particles are shown, and one in the middle moves. In the middle, many round light-blue shapes move amid a light-blue web. On the right, a snowman-shaped particle is engulfed by a light-blue sphere that fuses with smaller spheres, becoming brighter blue. Simultaneous imaging of phagosome rotation and phagosome-lysosome fusion in macrophage cells was achieved by using Förster Resonance Energy Transfer (FRET)-based particle sensors. As shown in the video on the left, each particle sensor is a “snowman”-shaped; the “snowman” body is a 1 m particle coated with FRET-donor fluorophore (red color), and the “snowman” head is a small fluorescent nanoparticle (green color) that is covalently attached. After the “snowman” shaped particle sensor was phagocytosed by a RAW 264.7 macrophage, it was encapsulated into a phagosome. As shown in the video in the middle, fusion between the sensor-containing phagosome and lysosomes loaded with FRET-acceptors generate real-time FRET signals (cyan, middle), which report the kinetics of phagosome-lysosome fusion. A merged video (on the right) shows both the particle sensor and FRET signals. More details can be found in Yu et al. bioRxiv 2021.04.04.438376; doi: https://doi.org/10.1101/2021.04.04.438376. Dear Abbey, Thank you for reaching out. We will be delighted to provide some videos for the gallery. Here is the link to a folder containing three videos and the corresponding word document with the necessary information. Yan Yu, Ph.D. Associate Professor Chemistry Department Indiana University 800 E. Kirkwood Ave Bloomington, IN 47401 Phone: (812) 855-0593 https://yu.lab.indiana.edu/
    KeywordsImmune system
    SourceYan Yu, Indiana University, Bloomington.
    Date
    Credit LineYanqi Yu, Yan Yu Group, Indiana University, Bloomington.
    Investigator
    Record TypeVideo
    Topic Area(s);#Cells;#Tools and Techniques;#
    Previous Uses
    StatusActive

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Phagosome-H.mp4
  
High1458 KB 1/21/2022 2:41 PMDolan, Lauren (NIH/NIGMS) [C]
Phagosome.mp4
  
Other1458 KB 1/20/2022 12:49 PMCrowley, Rachel (NIH/NIGMS) [E]
Phagosome.png
  
Thumbnail211 KB 1/20/2022 12:49 PMCrowley, Rachel (NIH/NIGMS) [E]

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