• 779737PublicAssets/6796
    Dividing yeast cells with spindle pole bodies and contractile rings
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    Dividing yeast cells with spindle pole bodies and contractile rings

    6796

    During cell division, spindle pole bodies (glowing dots) move toward the ends of yeast cells to separate copied genetic information. Contractile rings (glowing bands) form in cells’ middles and constrict to help them split. This time-lapse video was captured using wide-field microscopy with deconvolution.

    Related to images 6791, 6792, 6793, 6794, 6797, 6798, and video 6795.
    Public Note
    Internal NoteAlt text: Oblong yeast cells, each with two glowing dots inside. One dot moves toward each end of the cell. Bands form in the middle of the cells and constrict until the cells divide. Time-lapse imaging of wild-type fission yeast cells expressing the myosin regulatory light chain Rlc1 tagged with mNeonGreen and the spindle pole body protein Sid4 tagged with mNeonGreen. These proteins are used to mark key stages of cytokinesis including contractile ring formation, maturation, and constriction. Images were taken every 2 minutes, and each second of the video contains seven images. mNeonGreen intensity is highlighted with the Fire lookup table using FIJI/imageJ. Permission email: Hi Abbey, My name is Alaina and I am a researcher in Dr. Kathy Gould’s laboratory at Vanderbilt. I have provided 6 images and 2 movies along with the captions, titles and credits in a separate word document. I am linking to a dropbox file as it is a lot of files but I would be happy to send you the files in whatever way you need. Please let me know if you require any additional information or if you have any issues with the files. I can also confirm that all these images are free of copyright restrictions. link: https://www.dropbox.com/sh/50svqbrl7h9dcjp/AADal6XohuYY9jjahXgvG99ja?dl=0 Thank you! Alaina Willet, Ph.D. | Research Instructor | Dr. Kathy Gould's Lab Vanderbilt University | Cell & Developmental Biology 1161 21st Ave S, MCN B2309 | Nashville, TN 37232
    KeywordsMitosis, research organisms
    SourceAlaina Willet, Kathy Gould’s lab, Vanderbilt University.
    Date2022-01-13 00:00:00
    Credit LineAlaina Willet, Kathy Gould’s lab, Vanderbilt University.
    Investigator
    Record TypeVideo
    Topic Area(s);#Cells;#Tools and Techniques;#
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    StatusActive

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