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    Two mouse fibroblast cells
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    Two mouse fibroblast cells

    6789

    Two mouse fibroblasts, one of the most common types of cells in mammalian connective tissue. They play a key role in wound healing and tissue repair. This image was captured using structured illumination microscopy.
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    Internal NoteAlt text: Two round cells with threadlike projections extending from them. Preparation: DNA was labeled with DAPI, and mitochondria were labeled by expressing a mitochondrial protein, cox8a. 3D image stacks were acquired with a structured illumination microscope, and a 3D color projection was created by combining both channels into a single stack and using the Temporal Color-code plug-in in Fiji (Fiji Is Just ImageJ). Permission email: HI Abbey, Thank you for checking back. I would be happy for you to use this image and video for the NIGMS gallery. I own the copyright to this material. The text you requested is below followed by a Dropbox link to the files. Cheers, Dylan Image: Two mouse fibroblast cells Preparation: DNA was labeled with DAPI and mitochondria were labeled by expressing a mitochondrial protein, cox8a. 3D image stacks were acquired with a structured illumination microscope and a 3D color projection was created by combining both channels into a single stack and using the Temporal Color-code plugin in Fiji (Fiji Is Just ImageJ). Technique: Structured Illumination Microscopy (SIM) Video: DNA during cell death and cell division Specimen: DNA during cell death and cell division Preparation: This time-lapse shows two cells over a 2 hour period. The one on the bottom goes through programmed cell death and the one on the top goes through cell division. As such, the movie starts with two cells and, even though one dies, ends with two cells. Technique: Confocal https://www.dropbox.com/sh/deuy174kgfyqndu/AADeRA6j_i156q5755pX4KZfa?dl=0
    KeywordsMice, SIM, research organism
    SourceDylan T. Burnette, Vanderbilt University School of Medicine.
    Date2021-12-27 00:00:00
    Credit LineDylan T. Burnette, Vanderbilt University School of Medicine.
    Investigator
    Record TypePhotograph
    Topic Area(s);#Cells;#Tools and Techniques;#
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    StatusActive

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