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    DNA replication origin recognition complex (ORC)
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    DNA replication origin recognition complex (ORC)

    3597

    A study published in March 2012 used cryo-electron microscopy to determine the structure of the DNA replication origin recognition complex (ORC), a semi-circular, protein complex (yellow) that recognizes and binds DNA to start the replication process. The ORC appears to wrap around and bend approximately 70 base pairs of double stranded DNA (red and blue). Also shown is the protein Cdc6 (green), which is also involved in the initiation of DNA replication. Related to video 3307 that shows the structure from different angles. From a Brookhaven National Laboratory news release, "Study Reveals How Protein Machinery Binds and Wraps DNA to Start Replication."
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    Internal NoteThe light brown shading represents the structure obtained by cryo-EM. The ribbon diagram structures came from X-ray crystallography and were superimposed on the cryo-EM structure. The DNA is also superimposed--the cryo-EM data did not reveal the location of the DNA. See news release: http://www.bnl.gov/bnlweb/pubaf/pr/PR_display.asp?prID=1391&template=Today
    Keywords3D, structure
    SourceHuilin Li, Brookhaven National Laboratory
    Date
    Credit LineHuilin Li, Brookhaven National Laboratory, and Bruce Stillman, Cold Spring Harbor Laboratory
    InvestigatorHuilin Li; Bruce Stillman
    Record TypeIllustration
    Topic Area(s);#Genes;#Molecular Structures;#Tools and Techniques;#
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    StatusActive

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