• 51251217288125625641291308308116556PublicAssets/3509
    Neuron with labeled synapses
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    Neuron with labeled synapses

    3509

    In this image, recombinant probes known as FingRs (Fibronectin Intrabodies Generated by mRNA display) were expressed in a cortical neuron, where they attached fluorescent proteins to either PSD95 (green) or Gephyrin (red). PSD-95 is a marker for synaptic strength at excitatory postsynaptic sites, and Gephyrin plays a similar role at inhibitory postsynaptic sites. Thus, using FingRs it is possible to obtain a map of synaptic connections onto a particular neuron in a living cell in real time.
    Public Note
    Internal NoteHi Sharon, Here are the images and the captions. Hope this is ok. don -----Original Message----- From: Sharon (NIH/NIGMS) [C] Reynolds [mailto:sharon.reynolds@nih.gov] Sent: Monday, July 29, 2013 5:58 AM To: Donald Arnold Subject: RE: your neuron images--may we include some in our gallery? Thank you! Sharon Sharon Reynolds, ELS Freelance Writer/Editor (Contractor) National Institute on Deafness and Other Communication Disorders National Institute of General Medical Sciences Phone: 410-271-8297 ________________________________ From: Donald Arnold [darnold@dornsife.usc.edu] Sent: Sunday, July 28, 2013 2:54 PM To: Reynolds, Sharon (NIH/NIGMS) [C] Subject: Re: your neuron images--may we include some in our gallery? Hi Sharon, I have some other images that I can send you. I am currently out of the country and will be back aug. 5. I'll contact you then Don Sent from my iPhone On Jul 28, 2013, at 5:23 PM, "Reynolds, Sharon (NIH/NIGMS) [C]" > wrote: Dear Dr. Arnold, In tracking NIGMS-funded published research, I came across the fantastic image from your June 19 Neuron paper that was posted on the NIH Director's Blog (http://directorsblog.nih.gov/2013/07/25/watching-neuron-attachments-in-real-time/). Do you have any similar high-resolution images that were not published (and thus are free of copyright restrictions) that we could put into the NIGMS public domain image and video gallery? If you do have any to share, please let me know, and I can let you know a bit more about the resolution we need and other details (captions, etc.). Best regards, Sharon Sharon Reynolds, ELS Freelance Writer/Editor (Contractor) National Institute on Deafness and Other Communication Disorders National Institute of General Medical Sciences Phone: 410-271-8297
    Keywordsstructure
    SourceDon Arnold and Richard Roberts, University of Southern California.
    Date2013-08-25 00:00:00
    Credit LineJason Junge, University of Southern California.
    InvestigatorDon B. Arnold and Richard W. Roberts, USC; CBB Funding: R01GM-083898
    Principle Investigators:
    Don B. Arnold http://dornsife.usc.edu/arnold
    Richard W. Roberts http://chems.usc.edu/faculty_staff/roberts.htm
    Record TypePhotograph
    Topic Area(s);#Cells;#
    Previous Uses
    StatusActive

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Low114 KB 3/28/2019 4:18 PMConstantinides, Stephen (NIH/NIGMS) [C]
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