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This is a super-resolution LM image taken by Hiro Hakozaki and Masa Hoshijima of NCMIR. The image contains highlighted calcium channels in cardiac muscle using a technique called dSTORM. The microscope used in the NCMIR lab was built by Hiro Hakozaki.
12/23/2020 5:37:10 PM
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dSTORM_Cardiac1_L Low 131 KB 6/3/2016 3:27 PM aamishral2 (NIH/NIGMS) [C
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Spinal nerves are part of the peripheral nervous system. They run within the spinal column to carry nerve signals to and from all parts of the body. The spinal nerves enable all the movements we do, from turning our heads to wiggling our toes, control the movements of our internal organs, such as the colon and the bladder, as well as allow us to feel touch and location of our limbs.
12/23/2020 8:04:47 PM
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2/7/2022 3:43:55 PM
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12/21/2020 5:16:51 PM
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E. coli_M Medium 203 KB 3/13/2018 3:59 PM Constantinides, Stephen (NIH/NIGMS) [C
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2/3/2020 7:41:16 PM
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12/21/2020 5:16:04 PM
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Blood clot_M Medium 557 KB 9/18/2019 2:02 PM Machalek, Alisa (NIH/NIAMS) [E
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What looks like the gossamer wings of a butterfly is actually the retina of a mouse, delicately snipped to lay flat and sparkling with fluorescent molecules. The image is from a research project investigating the promise of gene therapy for glaucoma. It was created at an NIGMS-funded advanced microscopy facility that develops technology for imaging across many scales, from whole organisms to cells to individual molecules. <BR><BR> The ability to obtain high-resolution imaging of tissue as large as whole mouse retinas was made possible by a technique called large-scale mosaic confocal microscopy, which was pioneered by the NIGMS-funded National Center for Microscopy and Imaging Research. The technique is similar to Google Earth in that it computationally stitches together many small, high-resolution images. <BR><BR> More details: <BR><BR> Glaucoma is a progressive eye disease and the leading cause of irreversible blindness. It is characterized by the death of neurons in the retina called retinal ganglion cells. A number of studies over the past decade suggest that targeting these cells with gene therapy designed to prevent their death might slow the progression of glaucoma. <BR><BR> This study is investigating whether a non-disease-causing virus (adeno-associated virus serotype 2) can effectively deliver genes to retinal ganglion cells. The researchers introduced into the virus a gene for green fluorescent protein (GFP) so they could visualize how well the virus transduced the cells. <BR><BR> Two months after viral delivery of the fluorescent vector to the eyes of 7-month-old mice, the researchers examined the entire retinas of the subjects under a microscope. The ability to obtain high-resolution imaging of tissue as large as whole mouse retinas was made possible by a technique called large-scale mosaic confocal microscopy, which was pioneered by the NIGMS-funded National Center for Microscopy and Imaging Research. The technique is similar to Google Earth in that it computationally stitches together many small, high-resolution images. <BR><BR> The researchers observed GFP expression (yellow) in all parts of the retinal ganglion cells (blue), including the soma, axons and dendritic tree. These results suggest that a viral delivery system could deliver therapeutic genes to retinal ganglion cells for treating glaucoma and related diseases. <BR><BR> EQUIPMENT: Olympus FluoView™ FV1000 Confocal Microscope. Fluorophores: green fluorescent protein and Alexa Fluor 568. Non-glaucomatous DBA/2J-Gpnmb+ mice. <BR><BR> Reflecting on the work, the lead researcher [Keunyoung (“Christine”) Kim] says: “It is amazing to see intricate and artistically organized microscopic structures. … I encountered an entirely new world invisible to the naked eye—a galaxy of infinite secrets and endless potential for discovery.”
7/19/2023 8:25:17 PM
7/19/2023 8:25:17 PM
Type Name Media Type File Size Modified
Mouse retina-II NCMIR-lowresoln Low 172 KB 10/19/2016 11:45 AM Machalek, Alisa (NIH/NIAMS) [E
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The cerebellum is the brain's locomotion control center. Found at the base of your brain, the cerebellum is a single layer of tissue with deep folds like an accordion. People with damage to this region of the brain often have difficulty with balance, coordination and fine motor skills. <BR><BR> This image of a mouse cerebellum is part of a collection of such images in different colors and at different levels of magnification from the National Center for Microscopy and Imaging Research (NCMIR).
12/18/2020 8:15:56 PM
12/18/2020 8:15:56 PM
Type Name Media Type File Size Modified
5800_Cerebellum NCMIR 3_S Low 187 KB 3/29/2019 9:40 AM Constantinides, Stephen (NIH/NIGMS) [C
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This image shows the extracellular matrix (ECM) on the surface of a soleus (lower calf) muscle in light brown and blood vessels in pink. Near the bottom of the photo, a vessel is opened up to reveal red blood cells. Scientists know less about the ECM in muscle than in other tissues, but it's increasingly clear that the ECM is critical to muscle function, and disruption of the ECM has been associated with many muscle disorders. The ECM in muscles stores and releases growth factors, suggesting that it might play a role in cellular communication.
12/17/2020 4:40:53 PM
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Type Name Media Type File Size Modified
Soleus_muscle_L Low 92 KB 6/3/2016 3:40 PM aamishral2 (NIH/NIGMS) [C
The ECM in muscles stores and releases
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The extracellular matrix (ECM) is most prevalent in connective tissues but also is present between the stems (axons) of nerve cells, as shown here. Blue-colored nerve cell axons are surrounded by brown-colored, myelin-supplying Schwann cells, which act like insulation around an electrical wire to help speed the transmission of electric nerve impulses down the axon. The ECM is pale pink. The tiny brown spots within it are the collagen fibers that are part of the ECM.
12/17/2020 4:38:32 PM
12/17/2020 4:38:32 PM
Type Name Media Type File Size Modified
myelinating_axons_L Low 106 KB 6/3/2016 3:40 PM aamishral2 (NIH/NIGMS) [C
TEM 5: Soleus muscle ECM on the muscle surface
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The cerebellum is the brain's locomotion control center. Found at the base of your brain, the cerebellum is a single layer of tissue with deep folds like an accordion. People with damage to this region of the brain often have difficulty with balance, coordination and fine motor skills. <BR><BR> This image of a mouse cerebellum is part of a collection of such images in different colors and at different levels of magnification from the National Center for Microscopy and Imaging Research (NCMIR).
12/18/2020 8:04:56 PM
12/18/2020 8:04:56 PM
Type Name Media Type File Size Modified
Cerebellum NCMIR 6 thumbnail Thumbnail 162 KB 10/20/2016 9:53 AM Machalek, Alisa (NIH/NIAMS) [E
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The human immunodeficiency virus (HIV),shown here as tiny purple spheres, causes the disease known as AIDS (for acquired immunodeficiency syndrome). HIV can infect multiple cells in your body, including brain cells, but its main target is a cell in the immune system called the CD4 lymphocyte (also called a T-cell or CD4 cell).
12/23/2020 8:03:30 PM
12/23/2020 8:03:30 PM
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NCMIR_HIV_infected_cell High 254 KB 6/3/2016 3:27 PM aamishral2 (NIH/NIGMS) [C
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This image of the hippocampus was taken with an ultra-widefield high-speed multiphoton laser microscope. Tissue was stained to reveal the organization of glial cells (cyan), neurofilaments (green) and DNA (yellow). The microscope Deerinck used was developed in conjunction with Roger Tsien (2008 Nobel laureate in Chemistry) and remains a powerful and unique tool today (2012, almost 20 years after it was developed).
2/23/2021 3:50:22 PM
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This image appeared on the cover
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A genetic disorder of the nervous system, neurofibromatosis causes tumors to form on nerves throughout the body, including a type of tumor called an optic nerve glioma that can result in childhood blindness. The image was used to demonstrate the unique imaging capabilities of one of our newest (at the time) laser scanning microscopes and is of a wildtype (normal) mouse retina in the optic fiber layer. This layer is responsible for relaying information from the retina to the brain and was fluorescently stained to reveal the distribution of glial cells (green), DNA and RNA in the cell bodies of the retinal ganglion neurons (orange) and their optic nerve fibers (red), and actin in endothelial cells surrounding a prominent branching blood vessel (blue). By studying the microscopic structure of normal and diseased retina and optic nerves, we hope to better understand the altered biology of the tissues in these tumors with the prospects of developing therapeutic interventions.
12/23/2020 4:44:59 PM
12/23/2020 4:44:59 PM
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This image appeared on the cover of Nature
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The small intestine is where most of our nutrients from the food we eat are absorbed into the bloodstream. The walls of the intestine contain small finger-like projections called villi which increase the organ's surface area, enhancing nutrient absorption. It consists of the duodenum, which connects to the stomach, the jejenum and the ileum, which connects with the large intestine. Related to <a href="http://images.nigms.nih.gov/index.cfm?event=viewDetail&imageID=3389" target=_blank>image 3389</a> .
12/23/2020 8:05:19 PM
12/23/2020 8:05:19 PM
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The small intestine is where most
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Stained glomeruli in the kidney. The kidney is an essential organ responsible for disposing wastes from the body and for maintaining healthy ion levels in the blood. It works like a purifier by pulling break-down products of metabolism, such as urea and ammonium, from the blood stream for excretion in urine. The glomerulus is a structure that helps filter the waste compounds from the blood. It consists of a network of capillaries enclosed within a Bowman's capsule of a nephron, which is the structure in which ions exit or re-enter the blood in the kidney.
12/23/2020 8:07:21 PM
12/23/2020 8:07:21 PM
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Stained cross section of the mouse tail.
12/23/2020 8:12:46 PM
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Myelinated axons in a rat spinal root. Myelin is a type of fat that forms a sheath around and thus insulates the axon to protect it from losing the electrical current needed to transmit signals along the axon. The axoplasm inside the axon is shown in pink. Related to <a href="https://imagesadminprod.nigms.nih.gov/index.cfm?event=viewDetail&imageID=3397">image 3397</a>.
12/23/2020 8:16:48 PM
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Top view of myelinated axons in a rat spinal root. Myelin is a type of fat that forms a sheath around and thus insulates the axon to protect it from losing the electrical current needed to transmit signals along the axon. The axoplasm inside the axon is shown in pink. Related to <a href="https://imagesadminprod.nigms.nih.gov/index.cfm?event=viewDetail&imageID=3396">image 3396</a>.
12/23/2020 8:19:29 PM
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The cerebellum is the brain's locomotion control center. Every time you shoot a basketball, tie your shoe or chop an onion, your cerebellum fires into action. Found at the base of your brain, the cerebellum is a single layer of tissue with deep folds like an accordion. People with damage to this region of the brain often have difficulty with balance, coordination and fine motor skills. For a lower magnification, see image 3370. This image is part of the Life: Magnified collection, which was displayed in the Gateway Gallery at Washington Dulles International Airport June 3, 2014, to January 21, 2015. To see all 46 images in this exhibit, go to https://www.nigms.nih.gov/education/life-magnified/Pages/default.aspx.
11/22/2022 7:05:01 PM
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The small intestine is where most of our nutrients from the food we eat are absorbed into the bloodstream. The walls of the intestine contain small finger-like projections called villi which increase the organ's surface area, enhancing nutrient absorption. It consists of the duodenum, which connects to the stomach, the jejenum and the ileum, which connects with the large intestine. Related to <a href="http://images.nigms.nih.gov/index.cfm?event=viewDetail&imageID=3389" target=_blank>image 3390</a> .
10/14/2020 8:45:49 PM
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Multiphoton fluorescence image of HeLa cells with cytoskeletal microtubules (magenta) and DNA (cyan). Nikon RTS2000MP custom laser scanning microscope. See related images 3518,3519,3521,3522.
9/27/2020 3:31:22 AM
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Multiphoton fluorescence image of HeLa cells stained with the actin binding toxin phalloidin (red), microtubules (cyan) and cell nuclei (blue). Nikon RTS2000MP custom laser scanning microscope. See related images 3518,3519,3520,3522.
9/27/2020 3:39:33 AM
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Multiphoton fluorescence image of cultured HeLa cells with a fluorescent protein targeted to the Golgi apparatus (orange), microtubules (green) and counterstained for DNA (cyan). Nikon RTS2000MP custom laser scanning microscope. See related images 3518,3519,3520,3521.
9/27/2020 3:38:02 AM
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This photograph of kidney tissue was taken using fluorescent light microscopy. For a close-up version and more detailed description, see image <a href="https://imagesadminprod.nigms.nih.gov/Pages/DetailPage.aspx?imageID=677">3725</a>. Related to image <a href="https://imagesadminprod.nigms.nih.gov/Pages/DetailPage.aspx?imageID=663">3675</a>.
2/16/2021 11:12:30 PM
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This photograph of kidney tissue, taken using fluorescent light microscopy, shows a close-up view of part of image <a href="https://imagesadminprod.nigms.nih.gov/Pages/DetailPage.aspx?imageID=675">3732</a>. Kidneys filter the blood, removing waste and excessive fluid, which is excreted in urine. The filtration system is made up of components that include glomeruli (for example, the round structure taking up much of the image's center is a glomerulus) and tubules (seen in cross-section here with their inner lining stained green). Related to image <a href="https://imagesadminprod.nigms.nih.gov/Pages/DetailPage.aspx?imageID=663">3675</a> .
2/16/2021 11:08:39 PM
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Stained kidney tissue. The kidney is an essential organ responsible for disposing wastes from the body and for maintaining healthy ion levels in the blood. It also secretes two hormones, erythropoietin (EPO) and calcitriol (a derivative of vitamin D), into the blood. It works like a purifier by pulling break-down products of metabolism, such as urea and ammonium, from the blood stream for excretion in urine. Related to image <a href="https://imagesadminprod.nigms.nih.gov/Pages/DetailPage.aspx?imageID=677">3725</a>.
2/4/2020 7:58:53 PM
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The kidney is an essential organ
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Cross section of human skeletal muscle. Image taken with a confocal fluorescent light microscope.
12/1/2020 6:09:36 PM
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DNA (blue) and actin (red) in cultured fibroblast cells.
12/1/2020 6:03:48 PM
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Elastin is a fibrous protein in the extracellular matrix (ECM). It is abundant in artery walls like the one shown here. As its name indicates, elastin confers elasticity. Elastin fibers are at least five times stretchier than rubber bands of the same size. Tissues that expand, such as blood vessels and lungs, need to be both strong and elastic, so they contain both collagen (another ECM protein) and elastin. In this photo, the elastin-rich ECM is colored grayish brown and is most visible at the bottom of the photo. The curved red structures near the top of the image are red blood cells.
12/17/2020 4:39:43 PM
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Nodes of Ranvier are short gaps in the myelin sheath surrounding myelinated nerve cells (axons). Myelin insulates axons, and the node of Ranvier is where the axon is exposed to the extracellular environment, allowing for the transmission of action potentials at these nodes via ion flows between the inside and outside of the axon. The image shows a cross-section through the node, with the surrounding ECM encasing and supporting the axon shown in cyan.
12/17/2020 4:42:11 PM
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Nodes of Ranvier are short gaps in
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The photo shows a confocal microscopy image of perineuronal nets (PNNs), which are specialized extracellular matrix (ECM) structures in the brain. The PNN surrounds some nerve cells in brain regions including the cortex, hippocampus and thalamus. Researchers study the PNN to investigate their involvement stabilizing the extracellular environment and forming nets around nerve cells and synapses in the brain. Abnormalities in the PNNs have been linked to a variety of disorders, including epilepsy and schizophrenia, and they limit a process called neural plasticity in which new nerve connections are formed. To visualize the PNNs, researchers labeled them with Wisteria floribunda agglutinin (WFA)-fluorescein. Related to <a href="https://imagesadminprod.nigms.nih.gov/index.cfm?event=viewDetail&imageID=3742">image 3742</a>.
12/17/2020 5:33:10 PM
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TEM 5: Soleus muscle ECM on
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The photo shows a confocal microscopy image of perineuronal nets (PNNs), which are specialized extracellular matrix (ECM) structures in the brain. The PNN surrounds some nerve cells in brain regions including the cortex, hippocampus and thalamus. Researchers study the PNN to investigate their involvement stabilizing the extracellular environment and forming nets around nerve cells and synapses in the brain. Abnormalities in the PNNs have been linked to a variety of disorders, including epilepsy and schizophrenia, and they limit a process called neural plasticity in which new nerve connections are formed. To visualize the PNNs, researchers labeled them with Wisteria floribunda agglutinin (WFA)-fluorescein. Related to <a href="https://imagesadminprod.nigms.nih.gov/index.cfm?event=viewDetail&imageID=3741">image 3741</a>.
12/17/2020 5:35:24 PM
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This image mostly shows normal cultured epithelial cells expressing green fluorescent protein targeted to the Golgi apparatus (yellow-green) and stained for actin (magenta) and DNA (cyan). The middle cell is an abnormal large multinucleated cell. All the cells in this image have a Golgi but not all are expressing the targeted recombinant fluorescent protein.
2/4/2020 9:04:47 PM
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This image shows collagen, a fibrous protein that's the main component of the extracellular matrix (ECM). Collagen is a strong, ropelike molecule that forms stretch-resistant fibers. The most abundant protein in our bodies, collagen accounts for about a quarter of our total protein mass. Among its many functions is giving strength to our tendons, ligaments and bones and providing scaffolding for skin wounds to heal. There are about 20 different types of collagen in our bodies, each adapted to the needs of specific tissues
12/17/2020 4:37:24 PM
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There are about 20 different types of
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The extracellular matrix (ECM) is most prevalent in connective tissues but also is present between the stems (axons) of nerve cells. The axons of nerve cells are surrounded by the ECM encasing myelin-supplying Schwann cells, which insulate the axons to help speed the transmission of electric nerve impulses along the axons.
12/17/2020 4:39:07 PM
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Tongue One is a series of two, see image <a href="https://imagesadminprod.nigms.nih.gov/Pages/DetailPage.aspx?imageID=3716">5810</a>
12/18/2020 8:21:57 PM
12/18/2020 8:21:57 PM
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Microscopy image of tongue. One is a series of two, see image <a href="https://imagesadminprod.nigms.nih.gov/Pages/DetailPage.aspx?imageID=3722">5811</a>
12/18/2020 8:21:03 PM
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This image of the hippocampus was taken with an ultra-widefield high-speed multiphoton laser microscope. Tissue was stained to reveal the organization of glial cells (cyan), neurofilaments (green) and DNA (yellow). The microscope Deerinck used was developed in conjunction with Roger Tsien (2008 Nobel laureate in Chemistry) and remains a powerful and unique tool today (2012, almost 20 years after it was developed).
2/23/2021 3:50:22 PM
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A genetic disorder of the nervous system, neurofibromatosis causes tumors to form on nerves throughout the body, including a type of tumor called an optic nerve glioma that can result in childhood blindness. The image was used to demonstrate the unique imaging capabilities of one of our newest (at the time) laser scanning microscopes and is of a wildtype (normal) mouse retina in the optic fiber layer. This layer is responsible for relaying information from the retina to the brain and was fluorescently stained to reveal the distribution of glial cells (green), DNA and RNA in the cell bodies of the retinal ganglion neurons (orange) and their optic nerve fibers (red), and actin in endothelial cells surrounding a prominent branching blood vessel (blue). By studying the microscopic structure of normal and diseased retina and optic nerves, we hope to better understand the altered biology of the tissues in these tumors with the prospects of developing therapeutic interventions.
12/23/2020 4:44:59 PM
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A genetic disorder of the nervous system, neurofibromatosis causes tumors to form on nerves throughout the body, including a type of tumor called an optic nerve glioma that can
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The small intestine is where most of our nutrients from the food we eat are absorbed into the bloodstream. The walls of the intestine contain small finger-like projections called villi which increase the organ's surface area, enhancing nutrient absorption. It consists of the duodenum, which connects to the stomach, the jejenum and the ileum, which connects with the large intestine. Related to <a href="http://images.nigms.nih.gov/index.cfm?event=viewDetail&imageID=3389" target=_blank>image 3389</a> .
12/23/2020 8:05:19 PM
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Stained glomeruli in the kidney. The kidney is an essential organ responsible for disposing wastes from the body and for maintaining healthy ion levels in the blood. It works like a purifier by pulling break-down products of metabolism, such as urea and ammonium, from the blood stream for excretion in urine. The glomerulus is a structure that helps filter the waste compounds from the blood. It consists of a network of capillaries enclosed within a Bowman's capsule of a nephron, which is the structure in which ions exit or re-enter the blood in the kidney.
12/23/2020 8:07:21 PM
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Myelinated axons in a rat spinal root. Myelin is a type of fat that forms a sheath around and thus insulates the axon to protect it from losing the electrical current needed to transmit signals along the axon. The axoplasm inside the axon is shown in pink. Related to <a href="https://imagesadminprod.nigms.nih.gov/index.cfm?event=viewDetail&imageID=3397">image 3397</a>.
12/23/2020 8:16:48 PM
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Top view of myelinated axons in a rat spinal root. Myelin is a type of fat that forms a sheath around and thus insulates the axon to protect it from losing the electrical current needed to transmit signals along the axon. The axoplasm inside the axon is shown in pink. Related to <a href="https://imagesadminprod.nigms.nih.gov/index.cfm?event=viewDetail&imageID=3396">image 3396</a>.
12/23/2020 8:19:29 PM
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The cerebellum is the brain's locomotion control center. Every time you shoot a basketball, tie your shoe or chop an onion, your cerebellum fires into action. Found at the base of your brain, the cerebellum is a single layer of tissue with deep folds like an accordion. People with damage to this region of the brain often have difficulty with balance, coordination and fine motor skills. For a lower magnification, see image 3370. This image is part of the Life: Magnified collection, which was displayed in the Gateway Gallery at Washington Dulles International Airport June 3, 2014, to January 21, 2015. To see all 46 images in this exhibit, go to https://www.nigms.nih.gov/education/life-magnified/Pages/default.aspx.
11/22/2022 7:05:01 PM
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Type Name Media Type File Size Modified
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The human immunodeficiency virus (HIV),shown here as tiny purple spheres, causes the disease known as AIDS (for acquired immunodeficiency syndrome). HIV can infect multiple cells in your body, including brain cells, but its main target is a cell in the immune system called the CD4 lymphocyte (also called a T-cell or CD4 cell).
12/23/2020 8:03:30 PM
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NCMIR_HIV_infected_cell High 254 KB 6/3/2016 3:27 PM aamishral2 (NIH/NIGMS) [C
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Spinal nerves are part of the peripheral nervous system. They run within the spinal column to carry nerve signals to and from all parts of the body. The spinal nerves enable all the movements we do, from turning our heads to wiggling our toes, control the movements of our internal organs, such as the colon and the bladder, as well as allow us to feel touch and location of our limbs.
12/23/2020 8:04:47 PM
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NCMIR_human_spinal_nerve_L Low 30 KB 6/3/2016 3:27 PM aamishral2 (NIH/NIGMS) [C
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The small intestine is where most of our nutrients from the food we eat are absorbed into the bloodstream. The walls of the intestine contain small finger-like projections called villi which increase the organ's surface area, enhancing nutrient absorption. It consists of the duodenum, which connects to the stomach, the jejenum and the ileum, which connects with the large intestine. Related to <a href="http://images.nigms.nih.gov/index.cfm?event=viewDetail&imageID=3389" target=_blank>image 3390</a> .
10/14/2020 8:45:49 PM
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The small intestine is where most
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This is a super-resolution LM image taken by Hiro Hakozaki and Masa Hoshijima of NCMIR. The image contains highlighted calcium channels in cardiac muscle using a technique called dSTORM. The microscope used in the NCMIR lab was built by Hiro Hakozaki.
12/23/2020 5:37:10 PM
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This is a super-resolution LM image taken by Hiro Hakozaki and Masa Hoshijima of NCMIR
The image contains highlighted calcium channels in cardiac muscle using a technique called
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DNA (blue) and actin (red) in cultured fibroblast cells.
12/1/2020 6:03:48 PM
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Slide42_M High 76 KB 12/1/2020 1:03 PM Walter, Taylor (NIH/NIGMS) [C
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