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Anaphase is the critical step during mitosis when sister chromosomes are disjoined and directed to opposite spindle poles, ensuring equal distribution of the genome during cell division. In this image, one pair of sister chromosomes at the top was lost and failed to divide after chemical inhibition of polo-like kinase 1.
This image depicts chromosomes (blue) separating away from the spindle mid-zone (red). Kinetochores (green) highlight impaired movement of some chromosomes away from the mid-zone or the failure of sister chromatid separation (top). Scientists are interested in detailing the signaling events that are disrupted to produce this effect.
The image is a volume projection of multiple deconvolved z-planes acquired with a Nikon widefield fluorescence microscope.
This image was chosen as a winner of the 2016 NIH-funded research image call. The research that led to this image was funded by NIGMS. Related to
Robert Lera and Mark Burkard, University of Wisconsin-Madison
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